A yeast top-model sequenced
29 Jun 2012
In the 1990's, an isogenic family of yeast strains, the Saccharomyces cerevisiae CEN.PK strains, was developed by crossing different laboratory strains. Since then, this family and especially the CEN.PK113-7D strain, has become widely used in systems biology studies, as well as in metabolic and evolutionary engineering studies. Because of the relevance of S. cerevisiae CEN.PK113-7D as a model strain, a large group of academic and industrial researchers set out to perform a de novo sequencing, assembly and annotation of the genome of this strain. The group identified almost 3000 indels (insertion/deletions) compared to the reference genome of S. cerevisiae S288C. A number of genes relating to maltose metabolism and biotin biosynthesis were identified in CEN.PK133-7D that is absent in S288C. It appears that CEN.PK133-7D is not only related to laboratory strains, such as S288C, but also to industrial strains. This may explain the robust physiological performance of CEN.PK113-7D in industrial settings. The annotated genome of S. cerevisiae CEN.PK113-7D is available through http://cenpk.tudelft.nl
Nijkamp JF, van den Broek M, Datema E, de Kok S, Bosman L, Luttik MA, Daran-Lapujade P, Vongsangnak W, Nielsen J, Heijne WH, Klaassen P, Paddon CJ, Platt D, Kotter P, van Ham RC, Reinders MJ, Pronk JT, Ridder DD, Daran JM
De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology.
Microb Cell Fact 2012, 11:36, doi:10.1186/1475-2859-11-36