Advanced Course on Metabolomics for Microbial Systems Biology

General info

Date 

24 Oct 2011 - 28 Oct 2011 

Location

Delft University of Technology, the Netherlands

Website

www.bsdl-edu.bt.tudelft.nl/content/documents/Broch...

Keywords

Developments in rapid sampling methods, measurement techniques and modeling approaches for microbial systems.

Organiser

Biotechnological Sciences Delft Leiden (BSDL)

Description

The advanced course “Metabolomics for Microbial Systems Biology” aims at teaching state of the art methods of quantitative metabolomics to industrial and academic research professionals (i.e. MSc, PhD). The focus is to gain insights into the complex metabolic control of central carbon metabolism and connected product formation pathways in industrial micro-organisms.

The course covers recent developments in rapid sampling methods, measurement techniques and modeling approaches for microbial systems. The first two days are dedicated to experimental techniques, from steady-state analysis to stimulus response experiments. Proper sampling and sample handling procedures for reliable and reproducible metabolome analysis will be discussed and practiced. The main measurement techniques addressed are based on liquid or gas chromatographic separation, coupled to mass spectrometric detection (e.g. Isotope Dilution Mass Spectrometry, IDMS). Additionally new methods for the identification of yet unknown metabolites (peaks) will be presented.

The third and fourth day focus on theoretical and modeling aspects of Systems Biology. Approaches from network reconstruction, stoichiometric and thermodynamic network analysis and in-vivo kinetic modeling will be covered. 13C tracer methods will be discussed to extend the information content of stationary state as well as dynamic experiments.

The last day will be dedicated to future developments and advanced applications of quantitative metabolomics to tackle specific biological questions, whereby also other hierarchical levels of the cell will be taken into account.

For more information, fees and registration, click here.