Individual pathways, similar response
12 Jul 2012
For many organisms, glucose is the primary source of carbon and of energy. The availability of cellular glucose drives many key biological processes, including growth and metabolism. Even in simple organisms like yeast, the glucose regulatory system has evolved into a complex network of monitoring glucose levels and relaying information to relevant mechanisms in metabolism and gene expression. Although glucose regulation has been studied extensively with yeast (Saccharomyces cerevisiae) as a model organism, a detailed view on how the various pathways are connected, the pathways' underlying hierarchy and the contribution of individual components is still lacking.
Eva Apweiler (UMC Utrecht) and colleagues generated gene expression profiles of DNA deletion mutants under high glucose growth conditions and subsequently used the data to relate the deleted gene to all transcripts that exhibited a significant change in response to the deletion. Overall, their analysis shows that the glucose signalling pathways in yeast are highly interconnected. As a result, disruption of any individual pathway leads to one main transcriptional response that mimics either a high or a low glucose response. This response appears to be mainly mediated by regulation of the biosynthesis of storage carbohydrates.
Apweiler E, Sameith K, Margaritis T, Brabers N, van de Pasch L, Bakker LV, van Leenen D, Holstege FC, Kemmeren P
Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis
BMC Genomics 2012, 13(1):239