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Advanced NGS Course: RNA-seq data analysis (3th edition)

General info

30 Oct 2013 - 01 Nov 2013 
Leiden, Pieter de la Courtgebouw, Wassenaarsweg 52, Leiden
Next-generation sequencing, NGS, data analysis, RNA-seq, transcriptome
Hoen, 't Peter-Bram Dr.
Celia van Gelder
Hoen, 't Peter-Bram Dr.


NBIC and partner LUMC  is organizing a 3-day course on RNA-seq data analysis from October 30 – November 1st,  2013. This is an expert course for people with experience in NGS and a follow-up course on the general NBIC NGS data analysis course (which will be given from 28-30 August 2013 in Rotterdam). The course will consist of seminars and hands-on command line, Galaxy and R practicals and will focus on data preprocessing, quality control, alignment, visualization and statistics for differential transcript expression. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects.

Course topics: 

  • RNA-seq experimental approaches 
  • Alignment and de novo assembly
  • Statistics for differential gene expression 
  • eQTL analysis and allele specific expression
  • Variant calling and RNA editing
  • Small RNA profiling
  • Software for RNA-seq data analysis

Target audience
Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend. Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed. Seats have been reserved for participants in the FP7-funded project RD-Connect and Marie Curie European Training Network TranCYST.


More information and registration

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