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The power of RNA-seq

General info

Date 
05 Jun 2013 - 07 Jun 2013 
Location
Wageningen, the Netherlands
Website
http://www.nbic.nl/education/nbic-phd-school/enrol...
Keywords
RNA-seq, transcriptome, experimental design, statistics, data analysis, mapping, quantification, differential expression, interpretation, pathways
Organiser
Experimental Plant Sciences (EPS, Wageningen UR), the Bioinformatics Laboratory of Wageningen University & NBIC
Contact(s)
Edouard Severing
Harm Nijveen
Patrick Koks
Sandra Smit

Description

The Power of RNA-seq is an introductory course for researchers who want to use RNA-seq in their research project.

The course will focus on questions like:

  • Which questions can be addressed with RNA-seq?
  • How many samples and replicates do I need?
  • Which steps are involved in an RNA-seq experiment?
  • What is Differential Expression?
  • What can go wrong?

This is a 3-day course that will consist of lectures in the morning and extensive hands-on computer practicals in the afternoon. You'll learn about all aspects of RNA-seq during the morning lectures on NGS & RNA-seq theory, but also the context, applicability, power and expected results of RNA-seq experiments. During the practicals, you'll learn the basic steps an RNA-seq pipeline consist of, how to interpret your data and to put the results to use in your research project. We'll use Galaxy, R and webtools for this.

Teachers

  • Gabino Sanchez-Perez
  • Elio Schijlen
  • Edouard Severing
  • Paul Eilers
  • Marc van Driel
  • Sandra Smit
  • Marco Bink
  • Aalt-Jan van Dijk
  • Ole Madsen
  • Basten Snoek
  • Richard Immink
  • Henri van de Geest

topics:

  • experiental design
  • sequencing requirements
  • steps in RNA-seq data analysis (data quality control, transcript identification, quantification, differential  expression, interpretation)

target audience:

Researchers in Life Sciences ('biologists') starting with application of NextGen Sequencing & RNA-seq.

  • No previous NGS experience is needed
  • No command line or Linux computer experience is required
  • No R-experience is required

For more information, contact Patrick Koks

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